Hotspot

rpl32-trnL-UAG

SSC · 114504-115360 · 857 bp

Back to Neolamarckia

Pi 0.0187

MarkerSeek score 59.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 857
variable_sites: 16
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGAAATCTCTTTCTACCGGG	TCGAACCGAGATGCTTTAGC	52.4	50.0	57.8	58.1	982-1001 bp	1.0	71.1
rpl32-trnL-UAG_p2	GGGAAATCTCTTTCTACCGGG	TCCTAAGAGCAGCGTGTCTA	52.4	50.0	57.8	57.9	956-975 bp	1.0	70.8
rpl32-trnL-UAG_p3	GGGAAATCTCTTTCTACCGGG	AGCAGCGTGTCTACCAATTT	52.4	45.0	57.8	57.8	949-968 bp	1.0	70.3
rpl32-trnL-UAG_p4	GGGAAATCTCTTTCTACCGGG	CCTAAGAGCAGCGTGTCTAC	52.4	55.0	57.8	57.8	955-974 bp	1.0	70.2
rpl32-trnL-UAG_p5	GGGAAATCTCTTTCTACCGGG	CTAAGAGCAGCGTGTCTACC	52.4	55.0	57.8	57.8	954-973 bp	1.0	70.2