Hotspot

trnR-ACG-trnN-GUU

IRb · 109423-109986 · 564 bp

Back to Neolamarckia

Pi 0.00887

MarkerSeek score 63.10

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 564
variable_sites: 5
pi: 0.00887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	656 bp	1.0	77.6
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	660 bp	1.0	76.7
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	699 bp	1.0	76.4
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GGTTCGAATCCTACTTGGGG	55.0	55.0	57.8	57.7	648 bp	1.0	76.3
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TTGGTCGTAGGTTCGAATCC	55.0	50.0	57.8	57.3	657 bp	1.0	73.6