Hotspot

petG-trnW-CCA

LSC · 68035-68175 · 141 bp

Back to Neolamarckia

Pi 0.0146

MarkerSeek score 62.90

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 2
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CAATACAGGCGTGGTGATCA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	224-227 bp	1.0	81.7
petG-trnW-CCA_p2	CAATACAGGCGTGGTGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	246-249 bp	1.0	81.6
petG-trnW-CCA_p3	ACAATACAGGCGTGGTGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	247-250 bp	1.0	81.6
petG-trnW-CCA_p4	ACAATACAGGCGTGGTGATC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	225-228 bp	1.0	81.6
petG-trnW-CCA_p5	CAATACAGGCGTGGTGATCA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.0	57.9	210-213 bp	1.0	81.4