Hotspot

petA-psbJ

LSC · 64807-65798 · 992 bp

Back to Neolamarckia

Pi 0.0133

MarkerSeek score 64.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 13
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1125-1137 bp	1.0	78.7
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1108-1120 bp	1.0	77.8
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1039-1051 bp	1.0	77.7
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1041-1053 bp	1.0	77.7
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1122-1134 bp	1.0	77.6