Hotspot

ycf4-cemA

LSC · 62298-62917 · 620 bp

Back to Neolamarckia

Pi 0.0148

MarkerSeek score 68.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 9
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CTTGCGCGTACCAATTGAAG	ATAAAGGGGGAGTGAGTGCT	50.0	50.0	58.1	58.0	682-899 bp	1.0	80.8
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	ATAAAGGGGGAGTGAGTGCT	45.0	50.0	57.8	58.0	709-926 bp	1.0	80.4
ycf4-cemA_p3	TTTCTTGCGCGTACCAATTG	ATAAAGGGGGAGTGAGTGCT	45.0	50.0	57.7	58.0	685-902 bp	1.0	79.1
ycf4-cemA_p4	GCGCGTACCAATTGAAGTTT	ATAAAGGGGGAGTGAGTGCT	45.0	50.0	57.7	58.0	679-896 bp	1.0	79.1
ycf4-cemA_p5	CTTGCGCGTACCAATTGAAG	TAAAGGGGGAGTGAGTGCTT	50.0	50.0	58.1	58.3	681-898 bp	1.0	78.9