Hotspot

trnE-UUC-trnT-GGU

LSC · 32057-32796 · 740 bp

Back to Neolamarckia

Pi 0.0136
MarkerSeek score 61.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
740
variable_sites
10
pi
0.0136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AAGAGAGGTGTCCTGAACCA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 830-841 bp 1.0 80.5
trnE-UUC-trnT-GGU_p2 GCCTCCTTGAAAGAGAGGTG GAACCGATGACTTACGCCTT 55.0 50.0 57.9 58.0 840-851 bp 1.0 80.4
trnE-UUC-trnT-GGU_p3 AAGAGAGGTGTCCTGAACCA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 809-820 bp 1.0 80.0
trnE-UUC-trnT-GGU_p4 GCCTCCTTGAAAGAGAGGTG CCATGGCGTTACTCTACCAC 55.0 55.0 57.9 58.1 819-830 bp 1.0 80.0
trnE-UUC-trnT-GGU_p5 AAGAGAGGTGTCCTGAACCA TGGCGTTACTCTACCACTGA 50.0 50.0 57.9 58.1 806-817 bp 1.0 79.9