Hotspot

psbM-trnD-GUC

LSC · 30745-31644 · 900 bp

Back to Neolamarckia

Pi 0.00781

MarkerSeek score 63.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 7
pi: 0.00781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGAACTAGAATGAATAGTGCAGT	TTCAATTGGTCAGAGCACCG	37.5	50.0	57.4	58.5	1018-1033 bp	1.0	40.9
psbM-trnD-GUC_p2	GGAACTAGAATGAATAGTGCAGT	TTCAATTGGTCAGAGCACCG	39.1	50.0	56.1	58.5	1017-1032 bp	1.0	40.9
psbM-trnD-GUC_p3	CGTAGGAACTAGAATGAATAGTGC	TTCAATTGGTCAGAGCACCG	41.7	50.0	57.0	58.5	1021-1036 bp	1.0	40.9
psbM-trnD-GUC_p4	TTGACTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	35.0	50.0	53.0	58.5	1060-1075 bp	1.0	40.8
psbM-trnD-GUC_p5	TTTGACTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	33.3	50.0	53.9	58.5	1061-1076 bp	1.0	40.8