Hotspot

rps2-rpoC2

LSC · 16840-17081 · 242 bp

Back to Neolamarckia

Pi 0.0124

MarkerSeek score 45.40

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 242
variable_sites: 3
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCCTCACAAATTGCGAACAC	AGGTGCGTTCGATTGATTCA	50.0	45.0	57.9	57.9	1632-1644 bp	1.0	79.9
rps2-rpoC2_p2	CCCTTACGCTTTGCAGAGAT	AGGTGCGTTCGATTGATTCA	50.0	45.0	58.0	57.9	1071-1092 bp	1.0	79.8
rps2-rpoC2_p3	CTGCTAAATCGGGGTCACAA	AGGTGCGTTCGATTGATTCA	50.0	45.0	57.9	57.9	1550-1562 bp	1.0	79.5
rps2-rpoC2_p4	CCCTTACGCTTTGCAGAGAT	ATTGGATTGTTGCGAGCAGA	50.0	45.0	58.0	58.2	787-808 bp	1.0	79.4
rps2-rpoC2_p5	CCCTTACGCTTTGCAGAGAT	TCTGTTACCGAGCCGTCTTA	50.0	50.0	58.0	58.2	738-759 bp	1.0	79.4