Hotspot

rps16-trnQ-UUG

LSC · 6155-7613 · 1459 bp

Back to Neolamarckia

Pi 0.0118

MarkerSeek score 53.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1459
variable_sites: 17
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GATTCCCGCGTGATACACTT	CTTTTGACCCCACCCAATCA	50.0	50.0	58.1	58.0	2110-2120 bp	1.0	81.0
rps16-trnQ-UUG_p2	GATTCCCGCGTGATACACTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1824-1834 bp	1.0	80.6
rps16-trnQ-UUG_p3	GATTCCCGCGTGATACACTT	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.1	58.1	2379-2389 bp	1.0	80.4
rps16-trnQ-UUG_p4	GATTCCCGCGTGATACACTT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.1	57.9	2384-2394 bp	1.0	80.4
rps16-trnQ-UUG_p5	GAACAATTGATTCCCGCGTG	CTTTTGACCCCACCCAATCA	50.0	50.0	58.1	58.0	2128 bp	0.5	59.1