Hotspot

ndhD

SSC · 111705-112748 · 1044 bp

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Pi 0.00578
MarkerSeek score 50.90
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1044
variable_sites
6
pi
0.00578
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TGTGCGAGTTTTTGAGAACC AGGAGAAATAAGGTATATAGGTGGT 45.0 36.0 56.9 56.3 1113-1119 bp 1.0 40.9
ndhD_p2 TGTGCGAGTTTTTGAGAACCT AGGAGAAATAAGGTATATAGGTGGT 42.9 36.0 58.4 56.3 1113-1119 bp 1.0 40.9
ndhD_p3 TTGTGCGAGTTTTTGAGAACC AGGAGAAATAAGGTATATAGGTGGT 42.9 36.0 57.6 56.3 1114-1120 bp 1.0 40.9
ndhD_p4 GTGCGAGTTTTTGAGAACCT AGGAGAAATAAGGTATATAGGTGGT 45.0 36.0 56.6 56.3 1112-1118 bp 1.0 40.9
ndhD_p5 TGTGCGAGTTTTTGAGAACC GGAGAAATAAGGTATATAGGTGGT 45.0 37.5 56.9 55.0 1112-1118 bp 1.0 40.9