Hotspot

trnN-GUU-rpl32

SSC · 108892-109671 · 780 bp

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Pi 0.00533

MarkerSeek score 61.40

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 780
variable_sites: 4
pi: 0.00533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AGTCAGTTAACAGCCAACCG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.1	57.3	915-937 bp	1.0	52.0
trnN-GUU-rpl32_p2	ACGACCAGTCAGTTAACAGC	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.8	57.3	921-943 bp	1.0	51.8
trnN-GUU-rpl32_p3	AGGATTCGAACCTACGACCA	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.2	57.3	934-956 bp	1.0	51.7
trnN-GUU-rpl32_p4	AGTCAGTTAACAGCCAACCG	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	58.1	56.2	922-944 bp	1.0	51.4
trnN-GUU-rpl32_p5	GGATTCGAACCTACGACCAG	GCTTTTACTGCCTCTAAAGATCC	55.0	43.5	57.8	57.3	933-955 bp	1.0	51.3