Hotspot

petA-psbJ

LSC · 62262-63291 · 1030 bp

Back to Neofinetia

Pi 0.003

MarkerSeek score 63.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1030
variable_sites: 3
pi: 0.003
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGTCCAAGGCCTTCTGTTC	TATTCCGGGTTGGGTTCATC	55.0	50.0	58.2	57.3	1142-1167 bp	1.0	75.0
petA-psbJ_p2	GAGTCCAAGGCCTTCTGTTC	CTATTCCGGGTTGGGTTCAT	55.0	50.0	58.2	57.3	1143-1168 bp	1.0	74.9
petA-psbJ_p3	GTCCAAGGCCTTCTGTTCTT	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.7	57.3	1140-1165 bp	1.0	74.2
petA-psbJ_p4	GTCCAAGGCCTTCTGTTCTT	CTATTCCGGGTTGGGTTCAT	50.0	50.0	57.7	57.3	1141-1166 bp	1.0	74.2
petA-psbJ_p5	TTACGAGTCCAAGGCCTTCT	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.4	57.3	1146-1171 bp	1.0	73.6