Hotspot

trnV-UAC-trnM-CAU

LSC · 50691-50877 · 187 bp

Back to Neofinetia

Pi 0.00568

MarkerSeek score 60.30

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 1
pi: 0.00568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	CGTGTAAACGAGGTGCTCTA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.4	58.4	260-271 bp	1.0	75.3
trnV-UAC-trnM-CAU_p2	TTGAAAAACATTGGCGCACG	CCCGCCGTATGAAAGCAATA	45.0	50.0	58.8	58.4	278-289 bp	1.0	73.7
trnV-UAC-trnM-CAU_p3	CGTGTAAACGAGGTGCTCTA	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.4	57.3	259-270 bp	1.0	73.3
trnV-UAC-trnM-CAU_p4	CCTTTGAAAAACATTGGCGC	CCCGCCGTATGAAAGCAATA	45.0	50.0	57.1	58.4	281-292 bp	1.0	72.5
trnV-UAC-trnM-CAU_p5	CGTGTAAACGAGGTGCTCTA	TTTGAACCAATGACTCCCGC	50.0	50.0	57.4	58.8	275-286 bp	1.0	72.4