Hotspot

trnE-UUC-CWS93_pgt010

LSC · 32425-33267 · 843 bp

Back to Neofinetia

Pi 0.0217

MarkerSeek score 67.30

Primer available yes

Label trnE-UUC-CWS93_pgt010

Feature Metadata

label_name: trnE-UUC-CWS93_pgt010
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 17
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-CWS93_pgt010_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	889-947 bp	1.0	84.1
trnE-UUC-CWS93_pgt010_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	868-926 bp	1.0	83.6
trnE-UUC-CWS93_pgt010_p3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	865-923 bp	1.0	83.5
trnE-UUC-CWS93_pgt010_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	867-925 bp	1.0	83.2
trnE-UUC-CWS93_pgt010_p5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	886-944 bp	1.0	82.9