Hotspot

psbM-trnD-GUC

LSC · 30667-31811 · 1145 bp

Back to Neofinetia

Pi 0.00437

MarkerSeek score 58.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 5
pi: 0.00437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	CCATGCATATGGCTGGGATT	50.0	50.0	57.9	58.1	1272-1290 bp	1.0	79.3
psbM-trnD-GUC_p2	CAGCGCAGTAGCAATAAACG	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.9	57.9	1250-1268 bp	1.0	79.1
psbM-trnD-GUC_p3	TTGGCTGACTGTTTTGACGT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1329-1347 bp	1.0	77.8
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1277-1295 bp	1.0	77.8
psbM-trnD-GUC_p5	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1307-1325 bp	1.0	77.7