Hotspot

petN-psbM

LSC · 29716-30561 · 846 bp

Back to Neofinetia

Pi 0.00244

MarkerSeek score 63.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 2
pi: 0.00244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGGCATCATTCACATGGAT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.0	57.9	1036-1057 bp	1.0	81.5
petN-psbM_p2	AGGATAGGGGGCATCATTCA	CGTTTATTGCTACTGCGCTG	50.0	50.0	57.9	57.9	1043-1064 bp	1.0	80.4
petN-psbM_p3	GGGGCATCATTCACATGGAT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.0	58.3	979-1000 bp	1.0	80.2
petN-psbM_p4	GGGGCATCATTCACATGGAT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.0	58.3	1031-1052 bp	1.0	80.0
petN-psbM_p5	GGATAGGGGGCATCATTCAC	CGTTTATTGCTACTGCGCTG	55.0	50.0	57.8	57.9	1042-1063 bp	1.0	79.9