Hotspot

trnC-GCA-petN

LSC · 28799-29625 · 827 bp

Back to Neofinetia

Pi 0.00366

MarkerSeek score 60.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 3
pi: 0.00366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGCAAATCCTTTATCCCCGG	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	954-960 bp	1.0	80.1
trnC-GCA-petN_p2	GCAAATCCTTTATCCCCGGT	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	953-959 bp	1.0	80.1
trnC-GCA-petN_p3	GATGTAATTTGGCGGCATGG	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	995-1001 bp	1.0	79.5
trnC-GCA-petN_p4	GGATGTAATTTGGCGGCATG	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	996-1002 bp	1.0	79.5
trnC-GCA-petN_p5	ATCCTTTATCCCCGGTTCGA	GCTCTAAAGCCCACTCCTTC	50.0	55.0	58.2	58.0	949-955 bp	1.0	79.1