Hotspot

rpoC2

LSC · 16128-20285 · 4158 bp

Back to Neocinnamomum

Pi 0.000722

MarkerSeek score 54.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4158
variable_sites: 6
pi: 0.000722
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGCAACGTACGAATAGTCCC	TGGAACCAAGACCGAAATGG	50.0	50.0	57.4	58.1	257 bp	1.0	75.3
rpoC2_p2	GCAACGTACGAATAGTCCCT	TGGAACCAAGACCGAAATGG	50.0	50.0	57.4	58.1	256 bp	1.0	75.3
rpoC2_p3	TGATTGAATCCTCGCCTTGA	TGGAACCAAGACCGAAATGG	45.0	50.0	56.9	58.1	222 bp	1.0	71.3
rpoC2_p4	AAGTGATTGAATCCTCGCCT	TGGAACCAAGACCGAAATGG	45.0	50.0	56.9	58.1	225 bp	1.0	71.2
rpoC2_p5	AGTGATTGAATCCTCGCCTT	TGGAACCAAGACCGAAATGG	45.0	50.0	56.9	58.1	224 bp	1.0	71.2