Hotspot

clpP

LSC · 71754-73799 · 2046 bp

Back to Neocinnamomum

Pi 0.00122

MarkerSeek score 51.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2046
variable_sites: 5
pi: 0.00122
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCAAATTATGATTCGAAATGGAA	CCCATTGTTTGTTACGTTTCCA	26.1	40.9	52.8	57.7	2135-2143 bp	1.0	40.3
clpP_p2	TTCAAATTATGATTCGAAATGGA	CCCATTGTTTGTTACGTTTCCA	26.1	40.9	52.8	57.7	2136-2144 bp	1.0	40.3
clpP_p3	TCAAATTATGATTCGAAATGGAA	ACCCATTGTTTGTTACGTTTCC	26.1	40.9	52.8	57.7	2136-2144 bp	1.0	40.3
clpP_p4	TTCAAATTATGATTCGAAATGGA	ACCCATTGTTTGTTACGTTTCC	26.1	40.9	52.8	57.7	2137-2145 bp	1.0	40.3
clpP_p5	TCAAATTATGATTCGAAATGGAA	ACGTTTCCACATCAAAGTGA	26.1	40.0	52.8	55.5	2122-2130 bp	1.0	40.3