Hotspot

atpF

LSC · 11335-12625 · 1291 bp

Back to Neocinnamomum

Pi 0.000776

MarkerSeek score 55.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1291
variable_sites: 2
pi: 0.000776
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	AAAAGGGGGCGAAGTGATAC	45.0	50.0	57.4	57.9	1458-1460 bp	1.0	74.1
atpF_p2	ACTAATTTCGTCGGCTCGAA	GGGGGCGAAGTGATACAAAA	45.0	50.0	57.4	58.2	1454-1456 bp	1.0	73.7
atpF_p3	ACTAATTTCGTCGGCTCGAA	CAAAAACAAAAAGGGGGCGA	45.0	45.0	57.4	57.7	1466-1468 bp	1.0	72.5
atpF_p4	ACTAATTTCGTCGGCTCGAA	TCAAAAACAAAAAGGGGGCG	45.0	45.0	57.4	57.7	1467-1469 bp	1.0	72.5
atpF_p5	AATTTCGTCGGCTCGAATGG	AAAAGGGGGCGAAGTGATAC	50.0	50.0	59.0	57.9	1455-1457 bp	1.0	71.3