Hotspot

atpB

LSC · 54098-55594 · 1497 bp

Back to Neocinnamomum

Pi 0.00134

MarkerSeek score 45.60

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 4
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CAATAGGGGCGTGATTTGGT	TCAACTTGCCATCGGACATT	50.0	45.0	58.2	57.8	1690 bp	1.0	76.7
atpB_p2	CAATAGGGGCGTGATTTGGT	GATCAACTTGCCATCGGACA	50.0	50.0	58.2	58.3	1692 bp	1.0	76.2
atpB_p3	CAATAGGGGCGTGATTTGGT	ATCAACTTGCCATCGGACAT	50.0	45.0	58.2	57.6	1691 bp	1.0	74.9
atpB_p4	CAATAGGGGCGTGATTTGGT	TCAAGCGCTAACTCCCATTT	50.0	45.0	58.2	57.5	1734 bp	1.0	74.4
atpB_p5	CAATAGGGGCGTGATTTGGT	AGCGCTAACTCCCATTTCTT	50.0	45.0	58.2	57.2	1731 bp	1.0	72.0