Hotspot

rps15-ycf1

SSC · 125697-126022 · 326 bp

Back to Neocinnamomum

Pi 0.0046

MarkerSeek score 56.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 326
variable_sites: 3
pi: 0.0046
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCTTCTTCTTGCGGAATAACC	ACGTATGTATCCGCGATTCG	42.9	50.0	56.0	58.0	401-403 bp	1.0	56.1
rps15-ycf1_p2	CGGGATGAATGAATTTTTGACCA	ACGTATGTATCCGCGATTCG	39.1	50.0	57.9	58.0	381-383 bp	1.0	55.7
rps15-ycf1_p3	ACCGGGATGAATGAATTTTTGAC	ACGTATGTATCCGCGATTCG	39.1	50.0	57.9	58.0	383-385 bp	1.0	55.7
rps15-ycf1_p4	GGAATAACCGGGATGAATGA	ACGTATGTATCCGCGATTCG	45.0	50.0	54.9	58.0	389-391 bp	1.0	55.4
rps15-ycf1_p5	GGAATAACCGGGATGAATGAA	ACGTATGTATCCGCGATTCG	42.9	50.0	55.7	58.0	389-391 bp	1.0	54.0