Hotspot

rpl32-trnL-UAG

SSC · 115764-116369 · 606 bp

Back to Neocinnamomum

Pi 0.00275
MarkerSeek score 57.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
606
variable_sites
3
pi
0.00275
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAAAAGGGTATCAGGCTGCA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 739-751 bp 1.0 80.6
rpl32-trnL-UAG_p2 GAAAAGGGTATCAGGCTGCA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 740-752 bp 1.0 80.6
rpl32-trnL-UAG_p3 AAAAGGGTATCAGGCTGCAG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 738-750 bp 1.0 80.6
rpl32-trnL-UAG_p4 AAAAGGGTATCAGGCTGCAG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 739-751 bp 1.0 80.6
rpl32-trnL-UAG_p5 GAAAAGGGTATCAGGCTGCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 749-761 bp 1.0 79.7