Hotspot

ndhF-rpl32

SSC · 114614-115583 · 970 bp

Back to Neocinnamomum

Pi 0.00155
MarkerSeek score 61.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
970
variable_sites
3
pi
0.00155
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAGCATAAGTCCCAACCC TGCAGCCTGATACCCTTTTC 50.0 50.0 57.8 57.9 1127-1141 bp 1.0 78.5
ndhF-rpl32_p2 ACAAGCATAAGTCCCAACCC CTGCAGCCTGATACCCTTTT 50.0 50.0 57.8 57.9 1128-1142 bp 1.0 78.5
ndhF-rpl32_p3 GGCACAAGCATAAGTCCCAA TGCAGCCTGATACCCTTTTC 50.0 50.0 58.5 57.9 1130-1144 bp 1.0 76.6
ndhF-rpl32_p4 GGCACAAGCATAAGTCCCAA CTGCAGCCTGATACCCTTTT 50.0 50.0 58.5 57.9 1131-1145 bp 1.0 76.5
ndhF-rpl32_p5 CAAGCATAAGTCCCAACCCT TGCAGCCTGATACCCTTTTC 50.0 50.0 57.5 57.9 1126-1140 bp 1.0 76.1