Hotspot

trnP-UGG-psaJ

LSC · 68549-68899 · 351 bp

Back to Neocinnamomum

Pi 0.00285

MarkerSeek score 46.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 2
pi: 0.00285
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTCAGCGCATCTGGGAATAA	50.0	50.0	58.1	58.0	519 bp	1.0	79.1
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTCAGCGCATCTGGGAATAA	50.0	50.0	58.1	58.0	518 bp	1.0	79.1
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTCAGCGCATCTGGGAATAA	50.0	50.0	58.1	58.0	530 bp	1.0	79.1
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTCAGCGCATCTGGGAATAA	50.0	50.0	58.1	58.0	529 bp	1.0	79.1
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTCAGCGCATCTGGGAATAA	50.0	50.0	58.1	58.0	532 bp	1.0	79.1