Hotspot

petA-psbJ

LSC · 64495-65639 · 1145 bp

Back to Neocinnamomum

Pi 0.00806

MarkerSeek score 65.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 18
pi: 0.00806
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	CATATTCCGGATTGGGCTCA	45.0	50.0	58.2	57.4	1274-1287 bp	1.0	76.2
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	TATTCCGGATTGGGCTCATC	45.0	50.0	58.2	57.1	1272-1285 bp	1.0	73.9
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	TCATATTCCGGATTGGGCTC	45.0	50.0	58.2	57.1	1275-1288 bp	1.0	73.9
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	CATATTCCGGATTGGGCTCAT	45.0	47.6	58.2	57.9	1274-1287 bp	1.0	72.1
petA-psbJ_p5	ACGTGTCCAAGGTTTGTTGT	GTTCATATTCCGGATTGGGCT	45.0	47.6	58.2	58.1	1277-1290 bp	1.0	71.7