Hotspot

ndhC-trnV-UAC

LSC · 50786-52545 · 1760 bp

Back to Neocinnamomum

Pi 0.00123

MarkerSeek score 56.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1760
variable_sites: 4
pi: 0.00123
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACTCCGGAAATGAGGAATGC	GCACGTTGGGTCTTTGAAAC	50.0	50.0	58.0	58.2	1945-1946 bp	1.0	78.6
ndhC-trnV-UAC_p2	ACTCCGGAAATGAGGAATGC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	1881-1882 bp	1.0	78.3
ndhC-trnV-UAC_p3	CTTCACTAATCGGGGCCAAA	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	1965-1966 bp	1.0	77.9
ndhC-trnV-UAC_p4	ACTAATCGGGGCCAAAACTC	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	1961-1962 bp	1.0	77.9
ndhC-trnV-UAC_p5	CTTCACTAATCGGGGCCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1901-1902 bp	1.0	77.7