Hotspot

trnS-GCU-trnG-UCC

LSC · 7709-8583 · 875 bp

Back to Neocinnamomum

Pi 0.0029

MarkerSeek score 62.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 5
pi: 0.0029
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	GAACGAATCGCGCTTTTACC	45.0	50.0	58.0	58.2	940-951 bp	1.0	79.5
trnS-GCU-trnG-UCC_p2	TCAGAAGGAACGGAGAGAGG	GAACGAATCGCGCTTTTACC	55.0	50.0	58.2	58.2	998-1009 bp	1.0	78.4
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	GAACGAATCGCGCTTTTACC	50.0	50.0	57.6	58.2	926-937 bp	1.0	76.5
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	GAACGAATCGCGCTTTTACC	55.0	50.0	58.5	58.2	921-932 bp	1.0	75.7
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	AGAACGAATCGCGCTTTTAC	45.0	45.0	58.0	57.2	941-952 bp	1.0	74.9