Hotspot

psbA-trnK-UUU

LSC · 1553-1814 · 262 bp

Back to Neocinnamomum

Pi 0.00382

MarkerSeek score 55.60

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 262
variable_sites: 2
pi: 0.00382
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GTGCTTTCGCGTCTCTCTAA	GGCTTTTGGAGGGGTCTTTT	50.0	50.0	58.0	58.3	386-388 bp	1.0	78.2
psbA-trnK-UUU_p2	GTGCTTTCGCGTCTCTCTAA	CCTGTGCGATCAAAAATGCA	50.0	45.0	58.0	57.6	411-413 bp	1.0	77.6
psbA-trnK-UUU_p3	GTGCTTTCGCGTCTCTCTAA	CCCTGTGCGATCAAAAATGC	50.0	50.0	58.0	58.4	412-414 bp	1.0	77.5
psbA-trnK-UUU_p4	GTGCTTTCGCGTCTCTCTAA	GCCCTGTGCGATCAAAAATG	50.0	50.0	58.0	58.4	413-415 bp	1.0	77.5
psbA-trnK-UUU_p5	GTGCTTTCGCGTCTCTCTAA	TGCGATCAAAAATGCAAGCA	50.0	40.0	58.0	57.6	407-409 bp	1.0	76.9