Hotspot

rpl22

IRb · 84983-85204 · 222 bp

Back to Nematolepis

Pi 0.018

MarkerSeek score 37.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: IRb
length_bp: 222
variable_sites: 6
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAATTCCCTCAAGACCGGAG	TCCCGGGCATCTACCATTAT	55.0	50.0	58.0	58.0	807-880 bp	1.0	81.1
rpl22_p2	GCGCAATTTCTTTTCCGTCA	TCCCGGGCATCTACCATTAT	45.0	50.0	58.0	58.0	1149-1222 bp	1.0	80.7
rpl22_p3	TCGGCAAGAATATTGGGGTG	TCCCGGGCATCTACCATTAT	50.0	50.0	57.9	58.0	1012-1085 bp	1.0	80.7
rpl22_p4	CAATTCCCTCAAGACCGGAG	CCGGATCGAAATGTTGGCTA	55.0	50.0	58.0	58.1	1193-1266 bp	1.0	80.5
rpl22_p5	CAATTCCCTCAAGACCGGAG	GGAAGGGCTCCAATTGGTAG	55.0	55.0	58.0	57.9	1107-1180 bp	1.0	80.5