Hotspot

trnV-GAC-rps12

IRa · 139873-141652 · 1780 bp

Back to Nematolepis

Pi 0.00262
MarkerSeek score 59.80
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1780
variable_sites
7
pi
0.00262
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1876-1908 bp 1.0 76.9
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1880-1912 bp 1.0 76.9
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1875-1907 bp 1.0 76.9
trnV-GAC-rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1878-1910 bp 1.0 76.0
trnV-GAC-rps12_p5 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1861-1893 bp 1.0 75.4