Hotspot

rps15-ycf1

SSC · 125065-125421 · 357 bp

Back to Nematolepis

Pi 0.0205
MarkerSeek score 40.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
357
variable_sites
11
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACCGATCCACAGTTTTCT CCACGATTGAAAATTCGTTA 42.9 35.0 57.5 52.2 446 bp 1.0 42.0
rps15-ycf1_p2 TCAACCGATCCACAGTTTTCT CCACGATTGAAAATTCGTTAA 42.9 33.3 57.5 53.2 446 bp 1.0 42.0
rps15-ycf1_p3 ACCGATCCACAGTTTTCTTCT CCACGATTGAAAATTCGTTA 42.9 35.0 57.2 52.2 443 bp 1.0 42.0
rps15-ycf1_p4 ACCGATCCACAGTTTTCTTCT CCACGATTGAAAATTCGTTAA 42.9 33.3 57.2 53.2 443 bp 1.0 42.0
rps15-ycf1_p5 TCAACCGATCCACAGTTTTC CCACGATTGAAAATTCGTTA 45.0 35.0 56.0 52.2 446 bp 1.0 42.0