Hotspot

trnR-ACG-trnN-GUU

IRb · 109891-110506 · 616 bp

Back to Nematolepis

Pi 0.00434

MarkerSeek score 62.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 616
variable_sites: 4
pi: 0.00434
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	TAGGGTACTTCCGGGAACAG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.1	58.1	846-849 bp	1.0	78.7
trnR-ACG-trnN-GUU_p2	TAGGGTACTTCCGGGAACAG	GCTGTTAACTGACTGGTCGT	55.0	50.0	58.1	57.8	769-772 bp	1.0	78.4
trnR-ACG-trnN-GUU_p3	TAGGGTACTTCCGGGAACAG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.1	58.2	756-759 bp	1.0	78.3
trnR-ACG-trnN-GUU_p4	TAGGGTACTTCCGGGAACAG	TAGAATCTCCCGCTGTTCCT	55.0	50.0	58.1	57.8	814-817 bp	1.0	78.3
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	795-798 bp	1.0	78.2