Hotspot

ndhC-trnV-UAC

LSC · 51995-52447 · 453 bp

Back to Nematolepis

Pi 0.0207

MarkerSeek score 48.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 453
variable_sites: 14
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCGGAAATTCGAAATGCCAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	58.0	57.8	570-572 bp	1.0	80.6
ndhC-trnV-UAC_p2	TCTGGCCCTTTGTTAATCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	600-602 bp	1.0	79.6
ndhC-trnV-UAC_p3	ACTGCGGAAATTCGAAATGC	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.7	57.8	573-575 bp	1.0	78.6
ndhC-trnV-UAC_p4	TAATCGGGGCTAAAACTGCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	587-589 bp	1.0	77.9
ndhC-trnV-UAC_p5	GCGGAAATTCGAAATGCCAA	GAAGGTCTACGGTTCGAGTC	45.0	55.0	58.0	57.5	569-571 bp	1.0	77.8