Hotspot

psbC-trnS-UGA

LSC · 36457-36703 · 247 bp

Back to Nematolepis

Pi 0.0469

MarkerSeek score 79.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 247
variable_sites: 14
pi: 0.0469
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	343-393 bp	1.0	85.6
psbC-trnS-UGA_p2	CCTGTTCTTTCGATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.3	58.1	304-354 bp	1.0	84.9
psbC-trnS-UGA_p3	GCAGCAGGGTTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	289-339 bp	1.0	84.9
psbC-trnS-UGA_p4	CCTGTTCTTTCGATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.3	58.2	250-300 bp	1.0	84.4
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	381-431 bp	1.0	83.0