Hotspot

trnY-GUA-trnE-UUC

LSC · 31791-31849 · 59 bp

Back to Nematolepis

Pi 0.0339
MarkerSeek score 61.10
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
59
variable_sites
3
pi
0.0339
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CCAGCGTAGACATATTGCCA ATTCGACTTCCCCTGGGG 50.0 61.1 57.8 58.3 144 bp 1.0 61.7
trnY-GUA-trnE-UUC_p2 ACCAGCGTAGACATATTGCC ATTCGACTTCCCCTGGGG 50.0 61.1 57.8 58.3 145 bp 1.0 61.7
trnY-GUA-trnE-UUC_p3 CGAATTTACAGTCCGTCCCC ATTCGACTTCCCCTGGGG 55.0 61.1 58.4 58.3 123 bp 1.0 61.1
trnY-GUA-trnE-UUC_p4 ACGAATTTACAGTCCGTCCC ATTCGACTTCCCCTGGGG 50.0 61.1 57.6 58.3 124 bp 1.0 60.9
trnY-GUA-trnE-UUC_p5 CCAGCGTAGACATATTGCCA GATTCGACTTCCCCTGGGG 50.0 63.2 57.8 59.5 145 bp 1.0 60.0