Hotspot

rps2-rpoC2

LSC · 16532-16754 · 223 bp

Back to Nematolepis

Pi 0.015

MarkerSeek score 61.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 223
variable_sites: 5
pi: 0.015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GGTGCCATTCTAGGATTCCA	ACAGGGAATTTATTCGGGGG	50.0	50.0	57.0	57.3	423-424 bp	1.0	66.9
rps2-rpoC2_p2	CCTGCTTCCATCATCTCTTCC	ACAGGGAATTTATTCGGGGG	52.4	50.0	58.2	57.3	375-376 bp	1.0	66.1
rps2-rpoC2_p3	CATTTCCGAGTACCATGACCA	ACAGGGAATTTATTCGGGGG	47.6	50.0	57.7	57.3	405-406 bp	1.0	65.1
rps2-rpoC2_p4	GGTGCCATTCTAGGATTCCA	AACAGGGAATTTATTCGGGGG	50.0	47.6	57.0	58.0	424-425 bp	1.0	64.7
rps2-rpoC2_p5	AGGTGCCATTCTAGGATTCC	ACAGGGAATTTATTCGGGGG	50.0	50.0	56.7	57.3	424-425 bp	1.0	64.5