Hotspot

rpoA

LSC · 85430-86449 · 1020 bp

Back to Nelumbo

Pi 0.00392

MarkerSeek score 48.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 4
pi: 0.00392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AAAGATGAAATAATTCCAATGGA	CGTAACCCCTATGCCACATA	26.1	50.0	52.3	56.8	1164 bp	1.0	40.3
rpoA_p2	AAAAGATGAAATAATTCCAATGGA	CGTAACCCCTATGCCACATA	25.0	50.0	53.2	56.8	1165 bp	1.0	40.3
rpoA_p3	AAGATGAAATAATTCCAATGGAT	CGTAACCCCTATGCCACATA	26.1	50.0	52.1	56.8	1163 bp	1.0	40.3
rpoA_p4	AAAGATGAAATAATTCCAATGGA	CGTAACCCCTATGCCACATAA	26.1	47.6	52.3	57.5	1164 bp	1.0	40.3
rpoA_p5	AAAGATGAAATAATTCCAATGGAT	CGTAACCCCTATGCCACATA	25.0	50.0	53.0	56.8	1164 bp	1.0	40.3