Hotspot

ndhH-rps15

SSC · 131716-131819 · 104 bp

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Pi 0.0101

MarkerSeek score 62.90

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 104
variable_sites: 1
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	AACACCATGCATTGATGGGT	CGTCAACGGCTGCTAGTTTA	45.0	50.0	58.1	58.3	270-275 bp	1.0	78.9
ndhH-rps15_p2	AACACCATGCATTGATGGGT	CGGGAGCCAAAAACTCGTTA	45.0	50.0	58.1	58.5	195-200 bp	1.0	78.1
ndhH-rps15_p3	GAGTCGAAGAACACCATGCA	CGTCAACGGCTGCTAGTTTA	50.0	50.0	58.2	58.3	279-284 bp	1.0	77.7
ndhH-rps15_p4	TGAGTCGAAGAACACCATGC	CGTCAACGGCTGCTAGTTTA	50.0	50.0	58.2	58.3	280-285 bp	1.0	77.7
ndhH-rps15_p5	ATGCATTGATGGGTGGTGAG	CGTCAACGGCTGCTAGTTTA	50.0	50.0	58.2	58.3	264-269 bp	1.0	77.6