Hotspot

psaC-ndhE

SSC · 126148-126453 · 306 bp

Back to Nelumbo

Pi 0.0359

MarkerSeek score 71.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 306
variable_sites: 11
pi: 0.0359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	GCCAGCAATTGTTTCGTCAA	50.0	45.0	58.1	57.9	484-500 bp	1.0	79.7
psaC-ndhE_p2	CAGGCTCGTACACATTGAGT	GCCAGCAATTGTTTCGTCAA	50.0	45.0	57.6	57.9	444-460 bp	1.0	77.9
psaC-ndhE_p3	AGCTTTACATCCGTCCCAAG	GCCAGCAATTGTTTCGTCAA	50.0	45.0	57.6	57.9	490-506 bp	1.0	77.5
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	ATTGGGCCAGCAATTGTTTC	50.0	45.0	58.1	57.5	489-505 bp	1.0	76.9
psaC-ndhE_p5	GCTTTACATCCGTCCCAAGG	GCCAGCAATTGTTTCGTCAA	55.0	45.0	58.6	57.9	489-505 bp	1.0	75.7