Hotspot

rpl32-trnL-UAG

SSC · 121808-122878 · 1071 bp

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Pi 0.0188

MarkerSeek score 58.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1071
variable_sites: 20
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGACAAATAAATAATCAAACA	TCCTAAGAGCAGCGTGTCTA	28.0	50.0	56.6	57.9	1143-1144 bp	1.0	41.8
rpl32-trnL-UAG_p2	TGTGCGACAAATAAATAATCAAACA	AGCAGCGTGTCTACCAATTT	28.0	45.0	56.6	57.8	1136-1137 bp	1.0	41.8
rpl32-trnL-UAG_p3	TGTGCGACAAATAAATAATCAAACA	CCTAAGAGCAGCGTGTCTAC	28.0	55.0	56.6	57.8	1142-1143 bp	1.0	41.8
rpl32-trnL-UAG_p4	TGTGCGACAAATAAATAATCAAACA	CTAAGAGCAGCGTGTCTACC	28.0	55.0	56.6	57.8	1141-1142 bp	1.0	41.8
rpl32-trnL-UAG_p5	TGTGCGACAAATAAATAATCAAACA	TTTAGAAGATGCCGCCACTC	28.0	50.0	56.6	58.0	1193-1194 bp	1.0	41.7