Hotspot

trnN-GUU-ndhF

IRb · 116636-118301 · 1666 bp

Back to Nelumbo

Pi 0.00906

MarkerSeek score 54.80

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1666
variable_sites: 15
pi: 0.00906
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ACGACCAGTCAGTTAACAGC	CGGGCGAATCTCTTCTTACC	50.0	55.0	57.8	58.1	1797-1807 bp	1.0	78.7
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	CGGGCGAATCTCTTCTTACC	50.0	55.0	58.2	58.1	1810-1820 bp	1.0	78.7
trnN-GUU-ndhF_p3	GGATTCGAACCTACGACCAG	CGGGCGAATCTCTTCTTACC	55.0	55.0	57.8	58.1	1809-1819 bp	1.0	78.3
trnN-GUU-ndhF_p4	ACGACCAGTCAGTTAACAGC	ATCAAATATGTTGGGGGCGG	50.0	50.0	57.8	58.3	1814-1824 bp	1.0	77.5
trnN-GUU-ndhF_p5	AGGATTCGAACCTACGACCA	ATCAAATATGTTGGGGGCGG	50.0	50.0	58.2	58.3	1827-1837 bp	1.0	77.5