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rpl33-rps18

LSC · 74945-75455 · 511 bp

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Pi 0.0532

MarkerSeek score 89.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 26
pi: 0.0532
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 89.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATCCAAT	55.0	50.0	58.5	58.6	681-738 bp	1.0	76.0
rpl33-rps18_p2	CGACACAATACGCCTAGTCG	ATCAATTCGATCCCCCGATC	55.0	50.0	58.5	57.2	685-742 bp	1.0	74.9
rpl33-rps18_p3	CGACACAATACGCCTAGTCG	AATTCGATCCCCCGATCCAA	55.0	50.0	58.5	58.9	682-739 bp	1.0	74.2
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	TTCGATCCCCCGATCCAATT	55.0	50.0	58.5	58.9	680-737 bp	1.0	74.2
rpl33-rps18_p5	CGACACAATACGCCTAGTCG	GCAAACGCCTACGAAAAGAT	55.0	45.0	58.5	57.1	656-713 bp	1.0	74.2