Hotspot

psaJ-rpl33

LSC · 74199-74743 · 545 bp

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Pi 0.0189

MarkerSeek score 71.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 545
variable_sites: 10
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGGTCTTTGGCAGGTCTTTT	CACCGTTTCGAACACAGTTG	50.0	50.0	58.3	57.9	676-684 bp	1.0	79.9
psaJ-rpl33_p2	GGGTCTTTGGCAGGTCTTTT	AACACCGTTTCGAACACAGT	50.0	45.0	58.3	58.3	678-686 bp	1.0	78.4
psaJ-rpl33_p3	GGGTCTTTGGCAGGTCTTTT	ACACCGTTTCGAACACAGTT	50.0	45.0	58.3	58.3	677-685 bp	1.0	78.4
psaJ-rpl33_p4	GGGTCTTTGGCAGGTCTTTT	TAGAAATGCCCGGTGATTCC	50.0	50.0	58.3	57.7	703-711 bp	1.0	77.7
psaJ-rpl33_p5	AACATATCTCTCCGTGGCAC	CACCGTTTCGAACACAGTTG	50.0	50.0	57.4	57.9	719-727 bp	1.0	77.2