Hotspot

petN-psbM

LSC · 31260-32388 · 1129 bp

Back to Nelumbo

Pi 0.00713

MarkerSeek score 63.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1129
variable_sites: 8
pi: 0.00713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTTTCGCTCGTAGTATGGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	1237-1244 bp	1.0	68.6
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1284-1291 bp	1.0	68.5
petN-psbM_p3	TTCGCTCGTAGTATGGGGAA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1235-1242 bp	1.0	67.8
petN-psbM_p4	TTTCGCTCGTAGTATGGGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1236-1243 bp	1.0	67.8
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1223-1230 bp	1.0	66.9