Hotspot

trnC-GCA-petN

LSC · 30247-31169 · 923 bp

Back to Nelumbo

Pi 0.00558

MarkerSeek score 64.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 923
variable_sites: 5
pi: 0.00558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	CCCCATACTACGAGCGAAAG	45.0	55.0	57.9	57.9	1055-1082 bp	1.0	80.9
trnC-GCA-petN_p2	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	1008-1035 bp	1.0	80.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CCCCATACTACGAGCGAAAG	50.0	55.0	58.1	57.9	999-1026 bp	1.0	80.8
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	952-979 bp	1.0	80.8
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	TCCCCATACTACGAGCGAAA	45.0	50.0	57.9	58.2	1056-1083 bp	1.0	80.1