Hotspot

atpF-atpH

LSC · 14886-15341 · 456 bp

Back to Nelumbo

Pi 0.0175

MarkerSeek score 65.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 8
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	541 bp	1.0	80.8
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.9	58.0	569 bp	1.0	80.1
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	TGGACTGGTTGTGGCATTAG	50.0	50.0	58.0	57.8	546 bp	1.0	79.3
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGGACTGGTTGTGGCATTAG	50.0	50.0	57.9	57.8	574 bp	1.0	78.6
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	CTGGTTGTGGCATTAGCACT	50.0	50.0	58.0	58.5	542 bp	1.0	77.2