Hotspot

psbI-trnS-GCU

LSC · 9566-9732 · 167 bp

Back to Nelumbo

Pi 0.0242
MarkerSeek score 62.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
167
variable_sites
4
pi
0.0242
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAGGA TAAAGCGGCGGATTGCTAAT 55.0 45.0 58.9 58.0 256-258 bp 1.0 74.4
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAGGA ATTGGGAGAGATGGCTGAGT 55.0 50.0 58.9 58.1 280-282 bp 1.0 73.6
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAGGA ACGAGTTTTTGTACCGAGGG 55.0 50.0 58.9 57.8 229-231 bp 1.0 73.6
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAGGA CGAGTTTTTGTACCGAGGGT 55.0 50.0 58.9 57.8 228-230 bp 1.0 73.6
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAGGA ATGGCTGAGTGGACTAAAGC 55.0 50.0 58.9 57.6 270-272 bp 1.0 71.3