Hotspot

trnK-UUU-rps16

LSC · 4402-5301 · 900 bp

Back to Nelumbo

Pi 0.0067

MarkerSeek score 63.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 6
pi: 0.0067
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.9	58.0	1106-1116 bp	1.0	80.1
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.9	58.0	1105-1115 bp	1.0	80.1
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1023-1033 bp	1.0	79.3
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1022-1032 bp	1.0	79.3
trnK-UUU-rps16_p5	CAGTCGCGGTCTTACAAACT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1107-1117 bp	1.0	78.8