Hotspot

psbA-trnK-UUU

LSC · 1450-1828 · 379 bp

Back to Nelumbo

Pi 0.0761

MarkerSeek score 88.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 28
pi: 0.0761
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 88.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AACACCGAACCATCCAATGT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.7	57.9	603-761 bp	1.0	81.2
psbA-trnK-UUU_p2	ACGGTTTTCAGTGCTGGTTA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.7	57.9	579-737 bp	1.0	81.0
psbA-trnK-UUU_p3	TCAAAACACCGAACCATCCA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.6	57.9	607-765 bp	1.0	80.7
psbA-trnK-UUU_p4	AACACCGAACCATCCAATGT	CGGTTTGGGGAGGGATTTTT	45.0	50.0	57.7	58.4	572-730 bp	1.0	79.6
psbA-trnK-UUU_p5	ACGGTTTTCAGTGCTGGTTA	CGGTTTGGGGAGGGATTTTT	45.0	50.0	57.7	58.4	548-706 bp	1.0	79.4